3-D IHC: Mouse large intestine - Tubulin Beta 3 (TUBB3), CD326 (Ep-CAM), Helix NP™ Green

Formalin-fixed, 300 micron-thick mouse large intestine section was blocked, permeabilized and stained overnight with the following:

Green: Alexa Fluor® 647 anti-Tubulin Beta 3 (TUBB3) (clone AA10) -
Magenta: Alexa Fluor® 594 anti-mouse CD326 (Ep-CAM) Antibody (clone G8.8) -
Blue: Helix NP™ Green -

All antibodies were used at 5 µg/mL, except for anti-Tubulin Beta 3 (TUBB3) at 2.5 µg/mL. Tissues were optically cleared, then analyzed at 205 µm imaging depth on a confocal microscope.

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